ogdhl is a modifier of akt-dependent signaling and nf-κb functionogdhl akt-dependent信号是一个修饰符和nf-κb函数.pdfVIP

OGDHL Is a Modifier of AKT-Dependent Signaling and NF-kB Function 1 2 1 1 3 1,4 Tanusree Sen , Nilkantha Sen , Maartje G. Noordhuis , Rajani Ravi , T-C Wu , Patrick K. Ha , 1 1 David Sidransky , Mohammad Obaidul Hoque * 1 Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, 2 Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, 3 Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland, United States of America, 4 Milton J Dance Jr. Head and Neck Center, Greater Baltimore Medical Center, Baltimore, Maryland, United States of America Abstract Oxoglutarate dehydrogenase (OGDH) is the first and rate-limiting component of the multi-enzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration. The essential role of this complex is in the degradation of glucose and glutamate and the OGDHL gene (one component of OGDHC) is down-regulated by promoter hypermethylation in many different cancer types. These properties suggest a potential growth modulating role of OGDHL in cancer; however, the molecular mechanism through which OGDHL exerts its growth modulating function has not been elucidated. Here, we report that restoration of OGDHL expression in cervical cancer cells lacking endogenous OGDHL expression suppressed cell proliferation, invasion and soft agar colony formation in vitro. Knockdown of OGDHL expression in cervical cancer cells expressing endogenous OGDHL had the opposite effect. Forced expression