oligomeric status and nucleotide binding properties of the plastid atpadp transporter 1 toward a molecular understanding of the transport mechanism低聚物的地位和核苷酸的绑定属性质体atpadp运输车1分子传输机制的理解.pdfVIP

Oligomeric Status and Nucleotide Binding Properties of the Plastid ATP/ADP Transporter 1: Toward a Molecular Understanding of the Transport Mechanism ´ 1,2,3¤a 1,2,3 4,5,6,7¤b 1,2,3 Aurelien Deniaud , Pankaj Panwar , Annie Frelet-Barrand , Florent Bernaudat , ´ 1,2,3, Christine Ebel1,2,3, Norbert Rolland4,5,6,7, Eva Pebay-Peyroula1,2,3* Celine Juillan-Binard ´ 1 CEA, Institut de Biologie Structurale Jean-Pierre Ebel, Grenoble, France, 2 CNRS, Institut de Biologie Structurale, Grenoble, France, 3 Universite Joseph Fourier Grenoble 1, ´ ´ Institut de Biologie Structurale, Grenoble, France, 4 CNRS, Laboratoire de Physiologie Cellulaire Vegetale, UMR5168, Grenoble, France, 5 CEA, LPCV, Institut de ´ Recherches en Technologies et Sciences pour le Vivant, Grenoble, France, 6 Universite Joseph Fourier Grenoble 1, LPCV, 7 INRA, LPCV, UMR1200, Grenoble, France Abstract Background: Chloroplast ATP/ADP transporters are essential to energy homeostasis in plant cells. However, their molecular mechanism remains poorly understood, primarily due to the difficulty of producing and purifying functional recombinant forms of these transporters. Methodology/Principal Findings: In this work, we describe an expression and purification protocol providing good yields and efficient solubilization of NTT1 protein from Arabidopsis thaliana. By biochemic