oligonucleotide based magnetic bead capture of onchocerca volvulus dna for pcr pool screening of vector black flies基于寡核苷酸的磁珠捕获的盘尾属肠扭结向量黑蝇的dna聚合酶链反应池筛选.pdfVIP

Oligonucleotide Based Magnetic Bead Capture of Onchocerca volvulus DNA for PCR Pool Screening of Vector Black Flies 1 2 ´ ´ 1 ´ 3 4 Hemavathi Gopal , Hassan K. Hassan , Mario A. Rodrıguez-Perez , Laurent D. Toe , Sara Lustigman , Thomas R. Unnasch2* ´ ´ ´ ´ 1 Centro de Biotecnologıa Genomica, Instituto Politecnico Nacional, Reynosa, Tamaulipas, Mexico, 2 Department of Global Health, Global Health Infectious Disease Research Program, University of South Florida, Tampa, Florida, United States of America, 3 Multi-Disease Surveillance Centre, Ouagadougou, Burkina Faso, 4 Laboratory of Molecular Parasitology, Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, United States of America Abstract Background: Entomological surveys of Simulium vectors are an important component in the criteria used to determine if Onchocerca volvulus transmission has been interrupted and if focal elimination of the parasite has been achieved. However, because infection in the vector population is quite rare in areas where control has succeeded, large numbers of flies need to be examined to certify transmission interruption. Currently, this is accomplished through PCR pool screening of large numbers of flies. The efficiency of this process is limited by the size of the pools that may be screened, which is in turn determined by the constraints imposed by the biochemistry of the assay. The current method of DNA purification from pools of vector black flies relies upon silica adsorption. This method can be applied to screen pools containing a maximum of 50 individuals (from the Latin American vectors) or 100 individu