oligonucleotide frequencies of barcoding loci can discriminate species across kingdoms寡核苷酸频率条码位点能辨别物种的王国.pdfVIP

Oligonucleotide Frequencies of Barcoding Loci Can Discriminate Species across Kingdoms Antariksh Tyagi., Sumit K. Bag., Virendra Shukla, Sribash Roy., Rakesh Tuli¤* Center for Plant Molecular Biology, National Botanical Research Institute (Council of Scientific and Industrial Research), Lucknow, India Abstract Background: DNA barcoding refers to the use of short DNA sequences for rapid identification of species. Genetic distance or character attributes of a particular barcode locus discriminate the species. We report an efficient approach to analyze short sequence data for discrimination between species. Methodology and Principal Findings: A new approach, Oligonucleotide Frequency Range (OFR) of barcode loci for species discrimination is proposed. OFR of the loci that discriminates between species was characteristic of a species, i.e., the maxima and minima within a species did not overlap with that of other species. We compared the species resolution ability of different barcode loci using p-distance, Euclidean distance of oligonucleotide frequencies, nucleotide-character based approach and OFR method. The species resolution by OFR was either higher or comparable to the other methods. A short fragment of 126 bp of internal transcribed spacer region in ribosomal RNA gene was sufficient to discriminate a majority of the species using OFR. Conclusions/Significance: Oligonucleotide frequency range of a barcode locus can discriminate between species. Ability to discriminate species using very short DNA fragments may have wider applications in forensic and conservation studies. Citation: Tyagi A, Bag SK, Shukla V, Roy S, Tuli R (2010) Oligonucleotide Frequencies of Barcoding Loci Can Discriminate Species across Kingdoms. PLoS ONE 5(8): e12330. doi:10.1371/journal.pone.0012330 Editor: Klaus F. X. Mayer, GSF Research Center for Environment and Health, Germa