omeprazole blocks stat6 binding to the eotaxin-3 promoter in eosinophilic esophagitis cells奥美拉唑块stat6绑定eotaxin-3启动子的嗜酸性食管炎细胞.pdfVIP

Omeprazole Blocks STAT6 Binding to the Eotaxin-3 Promoter in Eosinophilic Esophagitis Cells 1 3 1 1 1 2 Xi Zhang , Edaire Cheng , Xiaofang Huo , Chunhua Yu , Qiuyang Zhang , Thai H. Pham , 1,4 1 1,4 David H. Wang , Stuart J. Spechler , Rhonda F. Souza * 1 Department of Internal Medicine, Veterans Affairs North Texas Health Care System and the University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, 2 Department of Surgery, Veterans Affairs North Texas Health Care System and the University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, 3 Department of Pediatrics, Children’s Medical Center and the University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, 4 Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America Abstract Background: Patients who have esophageal eosinophilia without gastroesophageal reflux disease (GERD) nevertheless can respond to proton pump inhibitors (PPIs), which can have anti-inflammatory actions independent of effects on gastric acid secretion. In esophageal cell cultures, omeprazole has been reported to inhibit Th2 cytokine-stimulated expression of eotaxin-3, an eosinophil chemoattractant contributing to esophageal eosinophilia in eosinophilic esophagitis (EoE). The objective of this study was to elucidate molecular mechanisms underlying PPI inhibition of IL-4-stimulated eotaxin-3 production by esophageal cells. Methods/Findings: Telomerase-immortalized and primary cultures of esophageal squamous cells from EoE patients were