omentum-wrapped scaffold with longitudinally oriented micro-channels promotes axonal regeneration and motor functional recovery in rats面向omentum-wrapped支架与纵向的微细血管促进轴突再生和在大鼠运动功能恢复.pdfVIP

Omentum-Wrapped Scaffold with Longitudinally Oriented Micro-Channels Promotes Axonal Regeneration and Motor Functional Recovery in Rats 1,2. 1. 1. 3 4 1 Yong-Guang Zhang , Jing-Hui Huang , Xue-Yu Hu , Qing-Song Sheng , Wei Zhao , Zhuo-Jing Luo * 1 Institution of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi’an, China, 2 476 Clinical Division, Fuzhou General Hospital, Fuzhou, China, 3 Department of Obstetrics and Gynecology, Xijing Hospital, Fourth Military Medical University, Xi’an, China, 4 Department of Biochemistry and Molecular Biology, Basic Medical Science College, Ningxia Medical University, Yinchuan, China Abstract Background: Tissue-engineered nerve scaffolds hold great potential in bridging large peripheral nerve defects. However, insufficient vascularization of nerve scaffolds limited neural tissues survival and regeneration, which hampered the successful implantation and clinical application of nerve scaffolds. The omentum possesses a high vascularization capacity and enhances regeneration and maturation of tissues and constructs to which it is applied. However, combined application of nerve scaffolds and omentum on axonal regeneration and functional recovery in the treatment of large peripheral nerve defects has rarely been investigated thus far. Methods: In the present study, an omentum-wrapped collagen-chitosan scaffold was used to bridge a 15-mm-long sciatic nerve defect in rats. Rats that received nerve autografts or scaffolds alone were served as positive control or negative control, respectively. The axonal regeneration and functional recovery were examined by a combination of walking track analysis, electrophys