on the effect of thermodynamic equilibrium on the assembly efficiency of complex multi-layered virus-like particles (vlp) the case of rotavirus vlp在热力学平衡的影响复杂的多层次的病毒样颗粒的组装效率(车牌区域)的情况下轮状病毒车牌区域.pdfVIP

On the Effect of Thermodynamic Equilibrium on the Assembly Efficiency of Complex Multi-Layered Virus-Like Particles (VLP): the Case of Rotavirus VLP ´ ˜ 1,2 1,2 3 1,2,4 1,2 Antonio Roldao , Maria Candida M. Mellado , J. C. Lima , Manuel J. T. Carrondo , Paula M. Alves , R. Oliveira2,3* ´ ´ ´ 1 Instituto de Tecnologia Quımica e Biologica-Universidade Nova de Lisboa (ITQB-UNL), Oeiras, Portugal, 2 Instituto de Biologia Experimental e Tecnologica (IBET), Oeiras, ˆ ˆ Portugal, 3 REQUIMTE, Faculdade de Ciencias e Tecnologia/Universidade Nova de Lisboa (FCT/UNL), Caparica, Portugal, 4 Faculdade de Ciencias e Tecnologia/ Universidade Nova de Lisboa (FCT/UNL), Monte de Caparica, Portugal Abstract Previous studies have reported the production of malformed virus-like-particles (VLP) in recombinant host systems. Here we computationally investigate the case of a large triple-layered rotavirus VLP (RLP). In vitro assembly, disassembly and reassembly data provides strong evidence of microscopic reversibility of RLP assembly. Light scattering experimental data also evidences a slow and reversible assembly untypical of kinetic traps, thus further strengthening the fidelity of a thermodynamically controlled assembly. In silico analysis further reveals that under favourable conditions particles distribution is dominated by structural