rhein reduces fat weight in dbdb mouse and prevents diet-induced obesity in c57bl6 mouse through the inhibition of pparγ signaling大黄酸减少脂肪重量dbdb鼠标和防止食源性肥胖在c57bl6小鼠pparγ信号的抑制.pdfVIP

Hindawi Publishing Corporation PPAR Research Volume 2012, Article ID 374936, 9 pages doi:10.1155/2012/374936 Research Article Rhein Reduces Fat Weight in db/db Mouse and Prevents Diet-Induced Obesity in C57Bl/6 Mouse through the Inhibition of PPARγ Signaling Yu Zhang,1 Shengjie Fan,1 Na Hu,2 Ming Gu,1 Chunxiao Chu,1 Yiming Li,1 Xiong Lu,2 and Cheng Huang1 1 School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China 2 Scientific Experimental Center, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China Correspondence should be addressed to Cheng Huang, chuang.shutcm@ Received 10 May 2012; Revised 24 July 2012; Accepted 7 August 2012 Academic Editor: Beata Lecka-Czernik Copyright © 2012 Yu Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Rheum palmatum has been used most frequently in the weight-reducing formulae in traditional Chinese medicine. However, the components of Rheum palmatum that play the antiobesity role are still uncertain. Here, we tested the weight-reducing effect of two major Rheum palmatum compounds on db/db mouse. We found that rhein (100 mg kg−1 day−1), but not emodin, reduced the fat weight in db/db mouse. Using diet-induced obese (DIO) C57BL/6 mice, we identified that rhein blocked high-fat diet- induced obesity, decreased fat mass and the size of white and brown adipocytes, and lowered serum cholesterol, LDL cholesterol, and fasting blood glucose levels in the mice. To elucidate the underlying mechanisms, we used reporter assay and gene expression analysis and found that rhein inhibited peroxisome proliferator-activated