screening antitumor compounds psoralen and isopsoralen from psoralea corylifolia l. seeds筛选抗肿瘤成分补骨脂素和isopsoralen补骨脂l .种子.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 363052, 7 pages doi:10.1093/ecam/nen087 Original Article Screening Antitumor Compounds Psoralen and Isopsoralen from Psoralea corylifolia L. Seeds Yi Wang, Chengtao Hong, Chenguang Zhou, Dongmei Xu, and Hai-bin Qu Department of Chinese Medicine Science Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China Correspondence should be addressed to Hai-bin Qu, quhb@ Received 12 August 2008; Accepted 16 December 2008 Copyright © 2011 Yi Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Psoralea corylifolia L. (Fabaceae) is a widely used medical plant in China. This study was designed to screen and identify bioactive compounds with anticancer activity from the seeds of Psoralea corylifolia L. One volatile fraction (fraction I) and three other fractions (fraction II, III, IV) from methanol extraction of P. corylifolia L. were obtained. Bioactivities of these fractions were evaluated by the cytotoxicity on KB, KBv200, K562, K562/ADM cancer cells with MTT assay. Major components in the active fraction were identified by HPLC/MSn . Fraction IV significantly inhibits the growth of cancer cells in a dose-dependent manner. The IC50 values were 21.6, 24.4, 10.0 and 26.9, respectively. Psoralen and isopsoralen, isolated from fraction IV, were subject to bioactive assay and presented a dose-dependent anticancer activity in four cancer cell lines (KB, KBv200, K562 and K562/ADM). The IC50 values of psoralen were 88.1, 86.6, 24.4 and 62.6, which of isopsoralen were 61.9, 4