screening of bioactive compounds from moutan cortex and their anti-inflammatory activities in rat synoviocytes生物活性化合物的筛选从牡丹在老鼠synoviocytes皮层及其抗炎活动.pdfVIP

Advance Access Publication 15 June 2007 eCAM 2009;6(1)57–63 doi:10.1093/ecam/nem066 Original Article Screening of Bioactive Compounds from Moutan Cortex and Their Anti-Inflammatory Activities in Rat Synoviocytes Mengjie Wu and Zhiyuan Gu Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Zhejiang University, Hangzhou 310006, China Moutan Cortex, a widely used traditional Chinese medicine for the treatment of various diseases, is the root bark of Paeonia suffruticosa Andrews (Paeoniaceae). Most of the pharmacological investigations of Moutan Cortex have been addressed to its central nervous system activities, anti-oxidative and sedative actions. Otherwise, there are few reports about the active compounds with anti-inflammatory activity of Moutan Cortex. The aim of the present study was to screen and identify bioactive compounds with anti-inflammatory effect from Moutan Cortex. With the aid of preparative high performance liquid chromatography (HPLC) technique, ethyl acetate and ethanol extract of Moutan Cortex were isolated into twenty-two fractions. Bioactivities of these fractions were evaluated by measuring expression of tumor necrosis factor-a (TNF-a) in rat synoviocytes subjected to interleukin-1b (IL-1b). Eight compounds were isolat