stat3 regulates proliferation and immunogenicity of the ewing family of tumors in vitrostat3调节增殖和肿瘤体外的尤因家族的免疫原性.pdfVIP

Hindawi Publishing Corporation Sarcoma Volume 2012, Article ID 987239, 6 pages doi:10.1155/2012/987239 Research Article STAT3 Regulates Proliferation and Immunogenicity of the Ewing Family of Tumors In Vitro Sam Behjati,1, 2 B. Piku Basu,1 Rebecca Wallace,1 Nelly Bier,2 Neil Sebire,1, 2 Fyeza Hasan,1 and John Anderson1, 2 1 Unit of Molecular Haematology and Cancer Biology, UCL Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK 2 Department of Histopathology, Great Ormond Street Hospital for Children NHS Trust, Great Ormond Street, London WC1N 3JH, UK Correspondence should be addressed to John Anderson, j.anderson@ich.ucl.ac.uk Received 15 August 2011; Revised 18 October 2011; Accepted 18 October 2011 Academic Editor: Luca Sangiorgi Copyright © 2012 Sam Behjati et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Ewing sarcoma family of tumors (ESFT) represents an aggressive spectrum of malignant tumour types with common defining histological and cytogenetic features. To evaluate the functional activation of signal transducer and activator of transcription 3 (STAT3) in ESFT, we evaluated its activation in primary tissue sections and observed the functional consequences of its inhibition in ESFT cell lines. STAT3 was activated (tyrosine 705-phosphorylated) in 18 out of 31 primary tumours (58%), either diffusely (35%) or focally (23%). STAT3 was constitutively activated in 3 out of 3 ESFT cell lines tested, and its specific chemical inhibition resulted in complete loss of cell viability. STAT3 inhibition in ESFT cell lines was associated with several consistent changes in chemok