the effect of bacterial adhesion on grafted chains revealed by the non-invasive sum frequency generation spectroscopy细菌粘附的影响显示接枝链的非侵入性和频率代光谱学.pdfVIP

Hindawi Publishing Corporation Spectroscopy: An International Journal Volume 27 (2012), Issue 5-6, Pages 571–579 doi:10.1155/2012/682591 The Effect of Bacterial Adhesion on Grafted Chains Revealed by the Non-Invasive Sum Frequency Generation Spectroscopy Emilie Bulard,1 Marie-Pierre Fontaine-Aupart,1 Henri Dubost,1 1 2 ¨ 2 Wanquan Zheng, Jean-Marie Herry, Marie-Noelle Bellon-Fontaine, Romain Briandet,2 and Bernard Bourguignon1 1 Institut des Sciences Moleculaires d’Orsay, ISMO-CNRS, Universite Paris Sud 11, ´ ´ 91405 Orsay, France 2 INRA AgroParisTech, UMR 1319, Micalis, 91300 Massy, France Correspondence should be addressed to Emilie Bulard, emilie.bulard@u-psud.fr Copyright © 2012 Emilie Bulard et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract. In biomedical and food industry, surface colonization by bacteria is harmful: it leads to biofilm formation, a microbial consortia more resistant to antibiotics than planktonic bacteria. In order to design materials able to limit the biofilm formation, the effect of bacteria on materials has to be well characterized. In this work, a well-defined surface composed of a self-assembled monolayer (SAM) of octadecanethiol (ODT) onto a gold surface is probed in situ. The SAM conformation is obtained using the femtosecond vibrational sum frequency generation (SFG) spectroscopy. This technique provides selectively the molecular vibrational signature of the interface. The behaviour of the ODT SAM is studied in different environments: in air, in water, and upon exposure to hydrophilic or hydrophobic Lactococcus lactis bacteria. Modell