synaptic structure and function in the mouse somatosensory cortex during chronic pain in vivo two-photon imaging在鼠标躯体感觉皮质突触结构和功能慢性疼痛体内双光子成像.pdfVIP

Hindawi Publishing Corporation Neural Plasticity Volume 2012, Article ID 640259, 8 pages doi:10.1155/2012/640259 Review Article Synaptic Structure and Function in the Mouse Somatosensory Cortex during Chronic Pain: In Vivo Two-Photon Imaging Sun Kwang Kim,1, 2 Kei Eto,1 and Junichi Nabekura1, 3, 4 1 Division of Homeostatic Development, National Institute for Physiological Sciences, Okazaki, Aichi 444-8585, Japan 2 Department of Physiology, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea 3 Department of Physiological Sciences, The Graduate School for Advanced Study, Hayama, Kanagawa 240-0193, Japan 4 Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan Correspondence should be addressed to Junichi Nabekura, nabekura@nips.ac.jp Received 23 August 2011; Accepted 13 November 2011 Academic Editor: Gunnar K. Gouras Copyright © 2012 Sun Kwang Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Recent advances in two-photon microscopy and fluorescence labeling techniques have enabled us to directly see the structural and functional changes in neurons and glia, and even at synapses, in the brain of living animals. Long-term in vivo two-photon imaging studies have shown that some postsynaptic dendritic spines in the adult cortex are rapidly eliminated or newly generated, in response to altered sensory input or synaptic activity, resulting in experience/activity-dependent rewiring of neuronal circuits. In vivo Ca2+ imaging studies have revealed the distinct, input-specific response patterns o