synchrotron ftir microspectroscopy study of the striatum in 6-hydroxydopamine rat model of parkinsons disease同步加速器6-hydroxydopamine鼠纹状体的红外光谱显微镜研究帕金森病模型.pdfVIP

Hindawi Publishing Corporation Spectroscopy: An International Journal Volume 27 (2012), Issue 4, Pages 229–238 doi:10.1155/2012/176937 Synchrotron FTIR Microspectroscopy Study of the Striatum in 6-Hydroxydopamine Rat Model of Parkinson’s Disease Zhu Hongyan,1 Pei Xiao,1 Wu Lingyan,1 Liu Bo,2 Qi Zeming,3 and Wang Yuyin3 1 Laboratory of Neuropharmacology and Neurotoxicology, School of Life Sciences, Shanghai University, Nanchen Road 333, Shanghai 200436, China 2 Shanghai Key Laboratory of Special Artificial Microstructure Materials and Technology, Physics Department, Tongji University, Shanghai 200092, China 3 National Synchrotron Radiation Laboratory, USTC, Hefei 230026, China Correspondence should be addressed to Zhu Hongyan, zhyred@ Copyright © 2012 Zhu Hongyan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract. In the present study, synchrotron-based Fourier transform-infrared (FTIR) microspectroscopy is used to analyze the biochemical composition of the striatal neurons in normal and Parkinson’s disease (PD) rat brain tissues. The rat model of Parkinson’s disease is established by destroying the nigrostriatal pathway with 6-hydroxydopamine (6-OHDA). The detailed spectral analyses show the significant changes of cellular compositions such as lipids, and proteins in the striatal neurons of 6-OHDA-lesioned PD rats with respect to control neurons. As a result, the intensities of spectral absorption assigned to lipid of the striatal neurons in PD rats are higher than in control animals. Furthermore, the unsaturation levels of phospholipids decrease in PD neurons with respect to control neurons, indicating a high level of lipid peroxidation. The analysis of protein secondary structure shows the significantly higher ratio of β-sheet in PD neurons compared