sphingosine 1-phosphate distribution in human plasma associations with lipid profiles鞘氨醇1-phosphate分布在人类血浆对血脂水平.pdfVIP

Hindawi Publishing Corporation Journal of Lipids Volume 2012, Article ID 180705, 8 pages doi:10.1155/2012/180705 Research Article Sphingosine 1-Phosphate Distribution in Human Plasma: Associations with Lipid Profiles Samar M. Hammad,1 Mohammed M. Al Gadban,1 Andrea J. Semler,2 and Richard L. Klein2, 3 1 Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC 29425, USA 2 Division of Endocrinology, Metabolism, and Medical Genetics, Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA 3 Research Service, Ralph H. Johnson VA Medical Center, United States Department of Veterans Aff airs, Charleston, SC 29401, USA Correspondence should be addressed to Samar M. Hammad, hammadsm@ and Richard L. Klein, kleinrl@ Received 8 August 2012; Accepted 27 September 2012 Academic Editor: Philip W. Wertz Copyright © 2012 Samar M. Hammad et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The physiological significance of sphingosine 1-phosphate (S1P) transport in blood has been debated. We have recently reported a comprehensive sphingolipid profile in human plasma and lipoprotein particles (VLDL, LDL, and HDL) using HPLC-MS/MS (Hammad et al., 2010). We now determined the relative concentrations of sphingolipids including S1P in the plasma subfraction containing lipoproteins compared to those in the remaining plasma proteins. Sphingomyelin and ceramide were predominantly recovered in the lipoprotein-containing fraction. Total plasma S1P concentration was positively correlated with S1P concentration in the protein-containing fracti