separation and identification of hsp-associated protein complexes from pancreatic cancer cell lines using 2d cnsds-page coupled with mass spectrometry分离和鉴定hsp-associated蛋白复合物从胰腺癌细胞系使用2 d cnsds-page加上质谱分析.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2011, Article ID 193052, 8 pages doi:10.1155/2011/193052 Research Article Separation and Identification of HSP-Associated Protein Complexes from Pancreatic Cancer Cell Lines Using 2D CN/SDSCoupled with Mass Spectrometry Zhiyun Zhao, Hui Liu, Xinli Wang, Xiaodong Wang, and Zhili Li Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing 100005, China Correspondence should be addressed to Zhili Li, lizhili@ Received 16 May 2011; Revised 12 August 2011; Accepted 17 August 2011 Academic Editor: Kapil Mehta Copyright © 2011 Zhiyun Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Protein complexes are a cornerstone of many biological processes and together they form various types of molecular machinery. A broad understanding of these protein complexes is crucial for revealing and building models of protein function and regulation. Pancreatic cancer is a highly lethal disease which is difficult to diagnose at early stage and even more difficult to cure. In this study, we applied a gradient clear native gel system combined with subsequent second-dimensional SDSto separate protein complexes from cell lysates of SW1990 and PANC-1 pancreatic cancer cell lines with different degrees of differentiation. Ten heat-shock-protein- (HSP-) associated protein complexes were separated and identified, and the differentially expressed proteins related to cancers were also found, such as HSP60, protein disulfide-isomerase A4 (ERp72