reverse genetics modification of cytomegalovirus antigenicity and immunogenicity by cd8 t-cell epitope deletion and insertion反向遗传学修饰巨细胞病毒抗原性和免疫原性的cd8 t细胞表位删除和插入.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2011, Article ID 812742, 15 pages doi:10.1155/2011/812742 Review Article Reverse Genetics Modification of Cytomegalovirus Antigenicity and Immunogenicity by CD8 T-Cell Epitope Deletion and Insertion Niels A. W. Lemmermann,1 Kai A. Kropp,1, 2 Christof K. Seckert,1 Natascha K. A. Grzimek,1 and Matthias J. Reddehase1 1 Institute for Virology, University Medical Center of the Johannes Gutenberg-University Mainz, Hochhaus am Augustusplatz, 55101 Mainz, Germany 2 Division of Pathway Medicine, Centre for Infectious Diseases, The University of Edinburgh, College of Medicine and Veterinary Medicine, The Chancellors Building, 49 Little France Crescent, Edinburgh, EH16 4SB, Scotland, UK Correspondence should be addressed to Niels A. W. Lemmermann, lemmermann@uni-mainz.de Received 4 August 2010; Accepted 27 October 2010 Academic Editor: Gregory Tannock Copyright © 2011 Niels A. W. Lemmermann et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The advent of cloning herpesviral genomes as bacterial artificial chromosomes (BACs) has made herpesviruses accessible to bacterial genetics and has thus revolutionised their mutagenesis. This opened all possibilities of reverse genetics to ask scientific questions by introducing precisely accurate mutations into the viral genome for testing their influence on the phenotype under study or to create phenotypes of interest. Here, we report on our experience with using BAC technology for a designed modulation of viral antigenicity and immunogenicity with focus o