non-centered spike-triggered covariance analysis reveals neurotrophin-3 as a developmental regulator of receptive field properties of on-off retinal ganglion cells明确spike-triggered协方差分析显示neurotrophin-3作为发展调节器开关视网膜神经节细胞的感受野特性.pdfVIP

Non-Centered Spike-Triggered Covariance Analysis Reveals Neurotrophin-3 as a Developmental Regulator of Receptive Field Properties of ON-OFF Retinal Ganglion Cells Donald R. Cantrell1,2, Jianhua Cang1,3, John B. Troy1,2*, Xiaorong Liu1,3* 1 Interdepartmental Neuroscience Program, Northwestern University, Evanston, Illinois, United States of America, 2 Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, 3 Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, United States of America Abstract The functional separation of ON and OFF pathways, one of the fundamental features of the visual system, starts in the retina. During postnatal development, some retinal ganglion cells (RGCs) whose dendrites arborize in both ON and OFF sublaminae of the inner plexiform layer transform into RGCs with dendrites that monostratify in either the ON or OFF sublamina, acquiring final dendritic morphology in a subtype-dependent manner. Little is known about how the receptive field (RF) properties of ON, OFF, and ON-OFF RGCs mature during this time because of the lack of a reliable and efficient method to classify RGCs into these subtypes. To address this deficiency, we developed an innovative variant of Spike Triggered Covariance (STC) analysis, which we term Spike Triggered Covariance – Non-Centered (STC-NC) analysis. Using a multi-electrode array (MEA), we recorded the responses of a large population of mouse RGCs to a Gaussian white noise stimulus. As expected, the Spike-Triggered Average (STA) fails to identify responses driven by symmetric static nonlinearities such as those that underlie ON-OFF center RGC behavior. The STC-NC technique, in contrast, provides an efficient means to identify ON-OFF responses and quantify their RF center sizes accurately. Using this