reversible oxidation of myometrial voltage-gated potassium channels with hydrogen peroxide可逆的氧化与过氧化氢子宫肌层的电压门控钾通道.pdfVIP

Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity Volume 2012, Article ID 105820, 7 pages doi:10.1155/2012/105820 Research Article Reversible Oxidation of Myometrial Voltage-Gated Potassium Channels with Hydrogen Peroxide Isabella Appiah,1 Aleksandra Nikolic-Kokic,1 Zorana Orescanin-Dusic,1 Ratko Radojicic,2 Slobodan Milovanovic,3 Mihajlo Spasic,1 and Dusko Blagojevic1 1 Department of Physiology, Institute for Biological Research “Sinisa Stankovic,” University of Belgrade, 11000 Belgrade, Serbia 2 Faculty of Biology, University of Belgrade, 11000 Belgrade, Serbia 3 Faculty of Medicine, University of Eastern Sarajevo, 73300 Foca, Bosnia and Herzegovina Correspondence should be addressed to Isabella Appiah, kuabena@ Received 18 April 2012; Accepted 8 August 2012 Academic Editor: David R. Jones Copyright © 2012 Isabella Appiah et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The uteri, spontaneously active or Ca2+ (6 mM) induced, were allowed to equilibrate, and to inhibit voltage-gated potassium (KV ) channels 1 mM 4-amino pyridine (4-AP) was applied for 15 min before adding H O . H O was added cumulatively: 2 μM, 20 μM, 2 2 2 2 200 μM, 400 μM, and 3 mM. Average time for H2 O2 concentrations (2, 20, 200, and 400) μM to reach its full effect was 15 min. H2 O2 3 mM had a prolonged effect and therefore was left to act for 30 min. Two-way ANOVA showed significant differences in time dependency between spontaneous and Ca2+ -induced rat uteri