sequence analysis of inducible prophage phis3501 integrated into the haemolysin ii gene of bacillus thuringiensis var israelensis atcc35646序列分析的诱导前噬菌体phis3501集成到溶血素ii基因的苏云金杆菌var israelensis atcc35646.pdfVIP

Hindawi Publishing Corporation Genetics Research International Volume 2012, Article ID 543286, 9 pages doi:10.1155/2012/543286 Research Article Sequence Analysis of Inducible Prophage phIS3501 Integrated into the Haemolysin II Gene of Bacillus thuringiensis var israelensis ATCC35646 Bouziane Moumen,1, 2 Christophe Nguen-The,2 and Alexei Sorokin1 1 UMR1319 Micalis, CRJ Institut National de la Recherche Agronomique, Bat. 440, Domaine de Vilvert, F-78352 Jouy-en-Josas, France 2 Securite et Qualite des Produits d’Origine Vegetale, UMR408, INRA Universite d’Avignon, 84914 Avignon Cedex 9, France ´ ´ ´ ´ ´ ´ Correspondence should be addressed to Bouziane Moumen, bouziane.moumen@jouy.inra.fr and Alexei Sorokin, alexei.sorokine@jouy.inra.fr Received 26 August 2011; Accepted 30 November 2011 Academic Editor: Tomaso Patarnello Copyright © 2012 Bouziane Moumen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Diarrheic food poisoning by bacteria of the Bacillus cereus group is mostly due to several toxins encoded in the genomes. One of them, cytotoxin K, was recently identified as responsible for severe necrotic syndromes. Cytotoxin K is similar to a class of proteins encoded by genes usually annotated as haemolysin II (hlyII) in the majority of genomes of the B. cereus group. The partially sequenced genome of Bacillus thuringiensis var israelensis ATCC35646 contains several potentially induced prophages, one of them integrated into the hlyII gene. We determined the complete sequence and established the genomic organization of this