synergistic cytotoxic effects of ganoderma lucidum and bacillus calmette guérin on premalignant urothelial huc-pc cells and its regulation on proinflammatory cytokine secretion协同细胞毒性的影响灵芝和杆菌calmette guerin癌变前的移行细胞huc-pc细胞及其对促炎细胞因子分泌的调节.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2012, Article ID 147896, 9 pages doi:10.1155/2012/147896 Research Article Synergistic Cytotoxic Effects of Ganoderma lucidum and Bacillus ´ Calmette Guerin on Premalignant Urothelial HUC-PC Cells and Its Regulation on Proinflammatory Cytokine Secretion John Wai-man Yuen,1 Mayur-Danny I. Gohel,2 and Chi-fai Ng3 1 School of Nursing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong 2 Department of Medical Science, Tung Wah College, Homantin, Hong Kong 3 Department of Surgery, The Chinese University of Hong Kong, Shatin, Hong Kong Correspondence should be addressed to John Wai-man Yuen, john.yuen@.hk Received 9 May 2012; Accepted 12 July 2012 Academic Editor: Jae Youl Cho Copyright © 2012 John Wai-man Yuen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ´ Bacillus Calmette-Guerin (BCG) is conventionally used as an adjuvant immunotherapy to reduce the recurrence of bladder cancer. To address the issues of efficacy and safety, an ethanol extract of Ganoderma lucidum (GLe) was evaluated for its interaction with BCG. In a model of premalignant human uroepithelial cells (HUC-PC), GLe exerted immediate cytotoxic effects while BCG showed a delayed response, given that both were immunological active in inducing the secretion of interleukin (IL)-6, IL-8, and monocyte chemotactic protein-1 (MCP-1). Synergistic cytotoxic effects were observed when cells were either coincubated with both drugs or firstly preincubated with GLe. Synergism between GLe and