spin ph and sh probes enhancing functionality of epr-based techniques自旋ph值和sh调查epr-based技术增强功能.pdfVIP

Spectroscopy 18 (2004) 213–225 213 IOS Press Spin pH and SH probes: enhancing functionality of EPR-based techniques Valery V. Khramtsov a,b,∗, Igor A. Grigor’ev c , David J. Lurie d , Margaret A. Foster d , Jay L. Zweier a and Periannan Kuppusamy a a Dorothy M. Davis Heart Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA b Institute of Chemical Kinetics Combustion, Novosibirsk 630090, Russia c Institute of Organic Chemistry, Novosibirsk 630090, Russia d Department of Biomedical Physics and Bioengineering, University of Aberdeen, Aberdeen, AB25 2ZD, UK Abstract. Along with significant progress in low-frequency electron spin resonance (ESR, also called electron paramagnetic resonance, EPR), other techniques such as Longitudinally-Detected ESR (LODESR), proton electron double-resonance imaging (PEDRI) and field-cycled dynamic nuclear polarization (FC-DNP) have been developed for in vivo applications. However their potential is still far from maximally defined, in part, because of the need for new specific function-directed spin probes. An application of stable nitroxides of imidazoline and imidazolidine types provides unique possibility to measure local values of pH and thiol content in various biological systems, including in vivo studies. These applications are based on the observation of specific chemical reactions of these nitroxides with protons or thiols, followed by significant changes in their EPR spectra. To increase sensitivity of pH probes for low-frequency EPR spectroscopy we evaluated two alternative approaches: (i) application of isotopically substituted labels, and (ii) acquisition of EPR spectra at high modulation amplitude. Spatial and spectral-spatial imaging (pH-mapping) using PEDRI and L-band EPR imagers were performed both on phantom samples and in vivo. The applications of the pH and SH probes in model system