sphingosine protects aging hearts from ischemiareperfusion injury superiority to sphingosine 1-phosphate and ischemic pre- and post-conditioning鞘氨醇保护老化的心从ischemiareperfusion伤害优势鞘氨醇1-phosphate和缺血性预处理和后置条件.pdfVIP

[Oxidative Medicine and Cellular Longevity 2:3, 146-151; July/August 2009]; ©2009 Landes Bioscience Research Paper Sphingosine protects aging hearts from ischemia/reperfusion injury Superiority to sphingosine 1-phosphate and ischemic pre- and post-conditioning 1,2, 1 1 3 Donald A. Vessey, * Michael Kelley, Luyi Li and Yong Huang 1Liver Study Unit; Department of Veterans Affairs Medical Center; San Francisco, CA USA; 2Department of Medicine and 3Drug Studies Unit; Department of Biopharmaceutical Sciences; University of California; San Francisco, CA USA Abbreviations: GPCR, G-protein coupled receptor; PKC, protein kinase C; PC, preconditioning; POST, postconditioning; IR, ischemia reperfusion; LVDP, left ventricular developed pressure; SKase, sphingosine kinase; S1P, sphingosine 1-phosphate; SD, standard deviation Key words: aging, Akt, cardioprotection, ischemia/reperfusion injury, preconditioning, postconditioning, sphingosine, sphingosine 1-phosphate Aging hearts are known to have diminished capacity to be cardioprotection in the old hearts. In conclusion, POST was less protected against reoxygenation ischemia/reperfusion (IR) injury affected by aging than PC; and sphingosine is a uniquely effective provided by various cardioprotective regimens. In search of a agent for both PC and POST of aging hearts. more successful regimen, we have studied the response of aged Introduction hearts to preconditioning (PC) and postconditioning (POST) elicited by sphingosine or sphingosine 1-phosphate treatment. The loss of coronary blood flow for an extended period of time An ex vivo rat heart model was used to stud