spectroscopic study of the interaction of dna with the linker histone h1 from starfish sperm reveals mechanisms of the formation of supercondensed sperm chromatin相互作用的光谱研究精子dna与海星的链接器组蛋白h1揭示supercondensed精子染色质的形成机制.pdfVIP

Hindawi Publishing Corporation Spectroscopy: An International Journal Volume 27 (2012), Issue 5-6, Pages 433–440 doi:10.1155/2012/250489 Spectroscopic Study of the Interaction of DNA with the Linker Histone H1 from Starfish Sperm Reveals Mechanisms of the Formation of Supercondensed Sperm Chromatin Elena Chikhirzhina,1 Tatyana Starkova,1, 2 Elena Kostyleva,1 and Alexander Polyanichko1, 2 1 Laboratory of Cell Biochemistry, Institute of Cytology, Russian Academy of Sciences, Saint-Petersburg 194064, Russia 2 Deprtment of Molecular Biophysics, Faculty of Physics, Saint-Petersburg State University, Saint-Petersburg 198504, Russia Correspondence should be addressed to Elena Chikhirzhina, chikhir@ Copyright © 2012 Elena Chikhirzhina et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract. The interaction of the linker histone H1Z from the sperm chromatin of starfish Asterias amurensis with DNA was studied by spectroscopic and thermodynamic approaches. It has been shown that at the physiological conditions the interaction of the H1Z with DNA results in more compact structures compared to complexes of DNA with somatic histone H1. The typical profile of the DNA melting curves reveals two peaks attributed to the bound and unbound DNA. It has been shown that H1Z from starfish sperm stabilizes DNA to a greater extent compared to the somatic H1. It is possible that the presence of the additional α—helical segments within the C-terminal part of the H1Z typical for the linker histones from echinoderm sperm facilitates the protein-protein interactions which in turn stimulate cooperative binding of the histones to DNA, resulting in the formation of the supercompact sperm chromatin. Keywords: Chromatin, circular cichroism, DNA, DNA melting, histone H1 1. Introduction Chromatin of euka