sphingosine kinase-1-dependent and -independent inhibitory effects of zanthoxyli fructus to attenuate the activation of mucosal mast cells and ameliorate food allergies in mice鞘氨醇kinase-1-dependent和独立的抑制性影响zanthoxyli果实减弱粘膜肥大细胞的激活和改善小鼠的食物过敏.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2012, Article ID 862743, 12 pages doi:10.1155/2012/862743 Research Article Sphingosine Kinase-1-Dependent and -Independent Inhibitory Effects of Zanthoxyli Fructus to Attenuate the Activation of Mucosal Mast Cells and Ameliorate Food Allergies in Mice Xiaoyu Wang, Natsuko Kageyama-Yahara, Shusaku Hayashi, Takeshi Yamamoto, and Makoto Kadowaki Division of Gastrointestinal Pathophysiology, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan Correspondence should be addressed to Makoto Kadowaki, makotok@inm.u-toyama.ac.jp Received 13 January 2012; Accepted 29 March 2012 Academic Editor: Olumayokun A. Olajide Copyright © 2012 Xiaoyu Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Food allergy (FA) is relatively a common disease in infants, but effective drug therapies are not yet available. Notably, mucosal mast cells, but not connective-tissue mast cells, play important roles in food allergic reactions via the release of inflammatory mediators. Therefore, we screened medicinal herb extracts for in vitro and in vivo antiallergic activity through inhibiting mucosal mast cell activation. As a result, both antigen-induced and calcium ionophore-induced degranulation was significantly inhibited by Zanthoxyli Fructus water extract (ZF) in mucosal-type murine bone marrow-derived mast cells (mBMMCs). ZF suppressed the antigen-induced [Ca2+ ] elevation and the antigen-enhanced mRNA expression of TNF-α, IL-4, and IL-13. The transcriptome and i