s-petasin, the main sesquiterpene of petasites formosanus, inhibits phosphodiesterase activity and suppresses ovalbumin-induced airway hyperresponsivenesss-petasin,主要的倍半萜烯petasites formosanus,抑制磷酸二酯酶活性和抑制ovalbumin-induced气道高反应性.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 132374, 14 pages doi:10.1093/ecam/nep088 Original Article S -Petasin, the Main Sesquiterpene of Petasites formosanus, Inhibits Phosphodiesterase Activity and Suppresses Ovalbumin-Induced Airway Hyperresponsiveness Chung-Hung Shih,1 Tzu-Jung Huang,2 Chien-Ming Chen,3 Yun-Lian Lin,4 and Wun-Chang Ko2 1 Department of Internal Medicine, Taipei Medical University Hospital, Taiwan 2 Graduate Institute of Pharmacology, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Department of Medical Technology, College of Medicine, Taipei Medical University, Taiwan 4 National Research Institute of Chinese Medicine, Taipei, Taiwan Correspondence should be addressed to Wun-Chang Ko, wc ko@.tw Received 30 March 2009; Accepted 15 June 2009 Copyright © 2011 Chung-Hung Shih et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. S-Petasin is the main sesquiterpene of Petasites formosanus, a traditional folk medicine used to treat hypertension, tumors and asthma in Taiwan. The aim of the present study was to investigate its inhibitory effects on phosphodiesterase (PDE) 1–5, and on ovalbumin (OVA)-induced airway hyperresponsiveness (AHR) in a murine model of allergic asthma. S-Petasin concentration-dependently inhibited PDE3 and PDE4 activities with 50% inhibitory concentrations (IC50 ) of 25.5, and 17.5 μM, respectively. According to the Lineweaver-Burk analysis, S-petasin competitively inhibited PDE3 and PDE4 activities with