synergistic effects between phosphorylation of phospholamban and troponin i promote relaxation at higher heart rate受磷和肌钙蛋白的磷酸化之间的协同作用促进放松在更高的心率.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2011, Article ID 651627, 10 pages doi:10.1155/2011/651627 Research Article Synergistic Effects between Phosphorylation of Phospholamban and Troponin I Promote Relaxation at Higher Heart Rate Lin Zhang, Yuan Yu, Zhen Song, Yun-Ying Wang, and Zhi-Bin Yu Department of Aerospace Physiology, Fourth Military Medical University, 169# Changlexi Road, Xi’an 710032, China Correspondence should be addressed to Zhi-Bin Yu, yuzhib@fmmu.edu.cn Received 13 February 2011; Accepted 17 June 2011 Academic Editor: Xupei Huang Copyright © 2011 Lin Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. We hypothesized that the extent of frequency-dependent acceleration of relaxation (FDAR) would be less than that of isoprot- erenol-(ISO-)dependent acceleration of relaxation (IDAR) at the same increment of heart rates, and ISO may improve FDAR. Cardiac function and phosphorylation of PLB and cTnI were compared in pacing, ISO treatment, and combined pacing and ISO treatment in isolated working heart. The increase in cardiac output and the degree of relaxation was less in pacing than in ISO treatment at the same increment of heart rates. The increasing stimulation frequency induced more significant relaxant effect in ISO perfusion than that in physiological salt perfusion. The pacing only phosphorylated PLB at Thr17, but ISO induced phosphorylation of cTnI and PLB at Ser16 and Thr17. Those results suggest that the synergistic effects of PLB and cTnI induce higher degree of relaxation which makes a sufficient diastolic filling of the ventricle at higher heart rate.