spectroscopic determination of pka constants of mads box segments光谱测定pka常量马德斯盒段.pdfVIP

Hindawi Publishing Corporation Spectroscopy: An International Journal Volume 27 (2012), Issue 5-6, Pages 455–461 doi:10.1155/2012/674032 Spectroscopic Determination of pKa Constants of MADS Box Segments ˇ ´ˇ ´ 1, 2 ¨ 3 2 Barbora Rezacova, Yves-Marie Coıc, Christian Zentz, 2 1 ˇ ˇ ´ Pierre-Yves Turpin, and Josef Stepanek 1 Faculty of Mathematics and Physics, Institute of Physics, Charles University in Prague, Ke Karlovu 5, 12116 Prague 2, Czech Republic 2 Laboratoire Acides Nucleiques et Biophotonique, FRE 3207, Universite Pierre et Marie Curie, ´ ´ place Jussieu 4, 75252 Paris Cedex 5, France 3 Unite de Chimie des Biomolecules, URA 2128 CNRS, Institut Pasteur, rue du Dr. Roux 28, ´ ´ 75724 Paris Cedex 15, France ˇ ´ˇ ´ Correspondence should be addressed to Barbora Rezacova, rezacova@karlov.mff.cuni.cz ˇ ´ˇ ´ Copyright © 2012 Barbora Rezacova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract. We have introduced a new promising approach for the determination of pKa constants of oligopeptide intrinsic fluorophores and spectral components referring to their differently charged states. The method is based on the factor analysis of multiwavelength spectroscopic pH titration data. As an illustrat