the effect of cordycepin on steroidogenesis and apoptosis in ma-10 mouse leydig tumor cells虫草素的影响类固醇生成和凋亡ma-10老鼠睾丸间质肿瘤细胞.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 750468, 14 pages doi:10.1155/2011/750468 Research Article The Effect of Cordycepin on Steroidogenesis and Apoptosis in MA-10 Mouse Leydig Tumor Cells Bo-Syong Pan, Chun-Yu Lin, and Bu-Miin Huang Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan Correspondence should be addressed to Bu-Miin Huang, bumiin@.tw Received 9 November 2010; Revised 25 January 2011; Accepted 30 March 2011 Copyright © 2011 Bo-Syong Pan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Cordycepin is a natural pure compound extracted from Cordyceps sinensis (CS). We have demonstrated that CS stimulates steroidogenesis in primary mouse Leydig cell and activates apoptosis in MA-10 mouse Leydig tumor cells. It is highly possible that cordycepin is the main component in CS modulating Leydig cell functions. Thus, our aim was to investigate the steroidogenic and apoptotic effects with potential mechanism of cordycepin on MA-10 mouse Leydig tumor cells. Results showed that cordycepin significantly stimulated progesterone production in dose- and time-dependent manners. Adenosine receptor (AR) subtype agonists were further used to treat MA-10 cells, showing that A , A , A , and A , AR agonists could stimulate progesterone 1 2A 2B 3 production. However, StAR promoter activity and protein expression remained of no difference among all cordycepin treatments, suggesting t