unique spectral features of dna infrared bands of some microorganisms独特的一些微生物的dna红外波段的光谱特征.pdfVIP

Spectroscopy 23 (2009) 291–297 291 DOI 10.3233/SPE-2009-0394 IOS Press Unique spectral features of DNA infrared bands of some microorganisms M. Khalique Ahmed ∗, Frank Amiama and Earl A. Sealy Department of Science, College of Arts and Sciences, Lynn University, Boca Raton, FL, USA Abstract. Half dozen microorganisms (Serratia marcescens, Klebsiella pneumonia, Staphylococcus aureus, Bacillus subtilis, Mycobacterium smegmatis and Saccharomyces cerevisiae) were cultivated, and their infrared spectra were recorded in the attenuated total reflection mode. Both the intensities and frequencies of the B-DNA and Z-DNA bands in the spectral region −1 of 1250–900 cm were found to be different for different microorganisms. We suggest that characterization and identification of microorganisms may be possible through analysis of their DNA bands only without the analysis of the full range (4000– −1 700 cm ) of their infrared spectra. Keywords: Microorganisms, attenuated total reflection, FT-IR, DNA, characterization, identification 1. Introduction The identification of microorganisms is important in the monitoring of the infectious diseases and in the understanding of the changes in the resistance patterns of the known microorganisms [9]. The tra- ditional methods of microorganism identification involve a variety of chemical tests [2,3,11,16] which are laborious and time intensive. In efforts to develop simple and efficient physiochemical instrumental methods for the identification of the microorganisms, several optical and chromatographic techniques have been reported [9]. Infrared and Raman spectral techniques have been shown to be useful in the identification and characterization of microorganisms [13]. Generally both infrared and Raman tech- niques utilize chemomatrics techniques that use all bands due to the different functi