identification of gene expression signature modulated by nicotinamide in a mouse bladder cancer model的基因表达信号调制识别烟酰胺在模型小鼠膀胱癌.pdfVIP

Identification of Gene Expression Signature Modulated by Nicotinamide in a Mouse Bladder Cancer Model 1,2¤ 1,2 3 4 5 1,2 Seon-Kyu Kim , Seok-Joong Yun , Jiyeon Kim , Ok-Jun Lee , Suk-Chul Bae , Wun-Jae Kim * 1 Department of Urology, Chungbuk National University College of Medicine, Cheongju, Korea, 2 BK21 Chungbuk Biomedical Science Center, Chungbuk National University School of Medicine, Cheongju, Korea, 3 Department of Pharmacology and Cancer Biology, Duke University Medical School, Durham, North Carolina, United States of America, 4 Department of Pathology, Chungbuk National University College of Medicine, Cheongju, Korea, 5 Department of Biochemistry, Chungbuk National University College of Medicine, Cheongju, Korea Abstract Background: Urinary bladder cancer is often a result of exposure to chemical carcinogens such as cigarette smoking. Because of histological similarity, chemically-induced rodent cancer model was largely used for human bladder cancer studies. Previous investigations have suggested that nicotinamide, water-soluble vitamin B3, may play a key role in cancer prevention through its activities in cellular repair. However, to date, evidence towards identifying the genetic alterations of nicotinamide in cancer prevention has not been provided. Here, we search for the molecular signatures of cancer prevention by nicotinamide using a N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN)-induced urinary bladder cancer model in mice. Methodology/Principal Findings: Via microarray gene expression profiling of 20 mice and 233 human bladder samples, we performed various statistical analyses and immunohist