identification of g1-regulated genes in normally cycling human cellsg1-regulated基因通常骑自行车人类细胞的识别.pdfVIP

Identification of G1-Regulated Genes in Normally Cycling Human Cells 1,4,5 1 2 3 4 Maroun J. Beyrouthy , Karen E. Alexander , Amy Baldwin , Michael L. Whitfield , Hank W. Bass , Dan 6 1 McGee , Myra M. Hurt * 1 Department of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, Florida, United States of America, 2 The Channing Laboratory, Brigham and Women’s Hospital and Department of Medicine, Harvard Medical School, Boston, Massachusetts, United States of America, 3 Department of Genetics, Norris Cotton Cancer Center, Dartmouth Medical School, Hanover, New Hampshire, United States of America, 4 Department of Biological Science, Florida State University, Tallahassee, Florida, United States of America, 5 Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, New Hampshire, United States of America, 6 Department of Statistics, Florida State University, Tallahassee, Florida, United States of America Abstract Background: Obtaining synchronous cell populations is essential for cell-cycle studies. Methods such as serum withdrawal or use of drugs which block cells at specific points in the cell cycle alter cellular events upon re-entry into the cell cycle. Regulatory events occurring in early G1 phase of a new cell cycle could have been overlooked. Methodology and Findings: We used a robotic mitotic shake-off apparatus to select cells in late mitosis for genome-wide gene expression studies. Two separate microarray experiments were conducted, one which involved isolation of RNA hourly for several hours from synchronous cell populations, and one experiment which examined gene activity every 15 minutes from late telophase of mitosis into G1 phase. To