identification of fluorescent compounds with non-specific binding property via high throughput live cell microscopy识别与非特异性荧光化合物的绑定属性通过高通量活细胞的显微镜.pdfVIP

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identification of fluorescent compounds with non-specific binding property via high throughput live cell microscopy识别与非特异性荧光化合物的绑定属性通过高通量活细胞的显微镜.pdf

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identification of fluorescent compounds with non-specific binding property via high throughput live cell microscopy识别与非特异性荧光化合物的绑定属性通过高通量活细胞的显微镜

Identification of Fluorescent Compounds with Non- Specific Binding Property via High Throughput Live Cell Microscopy 1 1 1 1 1 1 Sangeeta Nath , Virginia A. Spencer , Ju Han , Hang Chang , Kai Zhang , Gerald V. Fontenay , Charles 2 1 3 4 1 Anderson , Joel M. Hyman , Marit Nilsen-Hamilton , Young-Tae Chang , Bahram Parvin * 1 Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America, 2 Energy Biosciences Institute, University of California, Berkeley, California, United States of America, 3 Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa, United States of America, 4 Department of Chemistry and MedChem Program of Life Sciences Institute, National University of Singapore, and Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore, Republic of Singapore Abstract Introduction: Compounds exhibiting low non-specific intracellular binding or non-stickiness are concomitant with rapid clearing and in high demand for live-cell imaging assays because they allow for intracellular receptor localization with a high signal/noise ratio. The non-stickiness property is particularly important for imaging intracellular receptors due to the equilibria involved. Method: Three mammalian cell lines with diverse genetic backgrounds were used to screen a combinatorial fluorescence library via high throughput live cell microscopy for potential ligands with high in- and out-flux properties. The bin