identification of genes from the fungal pathogen cryptococcus neoformans related to transmigration into the central nervous system鉴定真菌病原体的基因相关的新型隐球菌轮回进入中枢神经系统.pdfVIP

Identification of Genes from the Fungal Pathogen Cryptococcus neoformans Related to Transmigration into the Central Nervous System 1,2,6 1 3 4 5 Hsiang-Kuang Tseng , Chang-Pan Liu , Michael S. Price , Ambrose Y. Jong , Jui-Chih Chang , 3 3 3 1,6 3 Dena L. Toffaletti , Marisol Betancourt-Quiroz , Aubrey E. Frazzitta , Wen-Long Cho *, John R. Perfect * 1 Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan, 2 Division of Infectious Diseases, Department of Internal Medicine, Mackay Memorial Hospital, Taipei, Taiwan, 3 Division of Infectious Diseases, Department of Medicine, Duke University Medical Center, Durham, NC, United States of America, 4 Division of Hematology-Oncology, The Saban Research Institute, Children’s Hospital Los Angeles, Department of Pediatrics, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America, 5 Division of Thoracic and Cardiovascular Surgery, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, 6 Department of Medicine, Mackay Medical College, New Taipei, Taiwan Abstract Background: A mouse brain transmigration assessment (MBTA) was created to investigate the central nervous system (CNS) pathogenesis of cryptococcal meningoencephalitis. Methodology/Principal Findings: Two cryptococcal mutants were identified from a pool of 109 pre-selected mutants that were signature-tagged with the nourseothricin acetyltransferase (NAT) resistance cassette. These two mutants displayed abnormal transmigra