不同诱导物对白腐菌XG8菌株产漆酶的影响-昆明理工大学学报.PDF

33 6 () http: / /www. kustjourna.l com/ Vo.l 33 No6 2008 12 Journal ofKunm ing niversity of Science andTechnology ( Science andTechnology) Dec. 2008 XG8 1 1 2 2 1 吴丽洁, 林连兵 , 陈克利, 岳凤霞 , 魏云林 ( 1. , 650224; 2. , 650224) : 利用木素溶液蔗渣乙醇及硫酸铜溶液分别对菌株XG8进行产漆酶诱导研究, 其中蔗渣 的诱导 果最明显.在静置培养时, 发酵液中漆酶的活力提高了 4倍; 当利用蔗渣乙醇及硫酸铜 同时进行诱导时, 漆酶活性与对照相比, 提高了 10倍.这些廉价的诱导物很适合应用于大规模的 工业化生产. : 白腐菌; 漆酶;诱导物; 蔗渣 : Q939. 97 : A : 1007- 855X( 2008) 06- 0094- 04 Effectof Several InducersonLaccase Production ofWhiteRotFungus Strain XG8 1 1 2 2 1 W U L-i j ie , L IN Lai n-b ing , CHEN Ke-li, YUE feng-x ai , W E IY un-lin ( 1. Biotechnology Research Center, Kunming niversity of Science and Technology, Kunm ing 650224, China; 2. Faculty ofBiologicaland Chem icalEngineering, Kunm ing niversity of Science and Technology, Kunm ing 650224, China) Abstract: Lignin, bagasse, ethanol and copper sulphate are used as inducers for fungus strain XG8 to produce laccase. It is shown through the results thatbagasse has thehighest induction efficiency than the other inducers. In static culture, the laccase activity is increased four tmi es. When induced w ith bagasse, ethanol and sulphate copper together, the laccase activity is ten tmi esof thatof the contro.l These inducers are quite suitable for indu- cingwhite rot fungus to produce laccase on a large scale for their low prices. ey words: white rot fungus; laccase; inducer; bagasse 0 , , [ 1] [2] , . , [3] ,, ,