生物导学案答案（Biology tutorial answer）.doc

生物导学案答案（Biology tutorial answer） Topic: the basic tool of DNA recombination technology [Preview 1] (read the contents of the textbook, complete the following questions and mark them in the corresponding positions in the book) First, the concept of genetic engineering Also called gene splicing techniques or recombinant DNA technology, genetic engineering, is in accordance with the wishes of the people, strict design, and through DNA recombination in vitro and transgenic technology to give biological genetic characteristics to create a new, more in line with the needs of the people of new biological types and biological products. [lesson 1] (combined with already learned knowledge, think about the following questions. The questions that can not be completed are drawn out in red, and discussed and communicated in the group, and the results are arranged on the study plan What are the basic biological principles that genetic engineering follows? Gene recombination (base complementation, pairing, genetic structure, sharing of codons, etc.) [preview 2] (read the contents of the textbook, complete the following questions and mark in the book) (random display) Two. Tools in genetic engineering 1. restriction endonuclease The restriction enzyme referred to as. (Pr) The main source is a kind of enzyme purification from prokaryotes to. The type: about 4000. The function: the identification of double stranded DNA molecules of a particular nucleotide sequence; between the two nucleotide specific parts of each strand of the phosphate ester bond breaking two. (specificity) At the end of the DNA after enzyme digestion: sticky end, flat end. 2.DNA ligase The role: two DNA fragments stitched up, restore the restriction enzyme cut up A two ester bond between two nucleotides. The type: E.coli DNA ligase and T4 DNA ligase. The 3. gene enters the receptor cell The necessary conditions: capable of self replication in the recipient cell, or integrated into chromosome DNA, Synchronous replic