生物试剂（Biological reagent）.docVIP

生物试剂（Biological reagent） 1 Fehling reagent detection of soluble reducing sugar Principle: sugar + Fehling reagent, brick red precipitate reduction Note: the Fehling reagent and the second liquid to a liquid mixture evenly before use, and is now equipped with water bath heating conditions, need. Application: Test and determine whether a sugar is a reducing sugar; determine the amount of sugar in different biological tissues; diagnose the disease in medicine, such as diabetes mellitus and nephritis. 2 Sultan III and Sultan IV detected fat Principle: Sultan III + fat + orange; Sultan IV + fat red Note: identification of fat requires microscopic observation. Application: test whether nutrients in foods contain fat. Detection of protein 3 biuret reagent Principle: protein + biuret reagent, purple Note: when using the biuret reagent, before adding A solution and B solution, reaction conditions for room temperature (without heating). Application: identifying proteins in certain digestive juices; used in identification of inferior milk powder. 4 iodine solution test starch Principle: starch + iodine liquid, blue Note: the iodine here is elemental iodine, not ionic iodine. Application: test whether food contains starch or not Staining and identification of 5DNA Dyeing principle: DNA+ methyl green to green Application: the distribution of DNA in cells can be displayed. Identification principle: DNA+ two aniline blue Application: an identification reagent used in DNA crude extraction experiment. 6, the red color of the RNA is red Principle: RNA+, red to red Application: the distribution of RNA in cells can be displayed. Note: when observing the distribution of DNA and RNA in cells, use a mixture of methyl green and piroxicam instead of individual staining. 7 trypan blue dyes dead cells blue Principle: the normal living cells, cell membrane integrity has selective permeability to trypan blue exclusion, so as not to enter the cytoplasm; dead cells or cell membrane intact cells,