科学仪器学 多功能酶标仪（Scientific instrument, multifunctional enzyme marker）.docVIP

科学仪器学 多功能酶标仪（Scientific instrument, multifunctional enzyme marker） The fluorescence analysis technology is a powerful analysis tool, widely used in clinical laboratory, biological research, agriculture, food and Environmental Sciences, is an important application of the multifunctional microplate, such as TECAN (M1000, M200), Thmeral (Varioskan Flash), Bio-tek (Synergy 4), MD (M2, M5) can be applied to fluorescence detection. 1. overview At room temperature, most of the molecules in the lowest vibrational levels of the ground state, in the ground state molecular absorption energy (solar energy, chemical energy, electricity or heat) after the jump moved to excited state, excited state is not stable, will soon decay to the ground state, releasing energy in the form of light, this phenomenon is called light phenomenon. Molecular luminescence includes fluorescence, phosphorescence, chemiluminescence, bioluminescence, etc.. By emitting light, light off when the luminescence disappeared immediately called fluorescence, light off when the light becomes weaker and disappear is called phosphorescence, chemical energy absorption and light chemical reaction called chemiluminescence, by biological energy into light radiation is known as bioluminescence. Because of the different luminescent materials, fluorescence has molecular fluorescence and atomic fluorescence, molecular fluorescence is band spectrum, atomic fluorescence is line spectrum, the fluorescence is usually molecular fluorescence. By measuring the characteristics and intensity of the emitted fluorescence, qualitative and quantitative analysis of the substance can be made. 2. fluorescence detection technique 2.1 fluorescence intensity (FI) The fluorescence intensity is in direct proportion to the concentration of the fluorescent material, which is the basis of quantitative analysis. The application in biology is very wide, can be quantitatively biomolecules, enzyme activity analysis, fluorescence immunoassay, cytolo