科学仪器学 紫外分光光度计(Scientific instrument, ultraviolet spectrophotometer).docVIP

科学仪器学 紫外分光光度计(Scientific instrument, ultraviolet spectrophotometer).doc

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科学仪器学 紫外分光光度计(Scientific instrument, ultraviolet spectrophotometer)

科学仪器学 紫外分光光度计(Scientific instrument, ultraviolet spectrophotometer) I. General principles of spectrophotometer Spectrophotometer is the qualitative and quantitative analysis of the substance by measuring the absorbance of the matter at a specific wavelength or a certain wavelength range by spectrophotometry. The basic principles of different types of spectrophotometer are similar to the use of a light source that produces multiple wavelengths, through a series of light splitting devices, resulting in a specific wavelength of light source. After the light source passes through the tested sample, a part of the light source is absorbed. By measuring the light absorption value of the sample, the calculation can be converted to the concentration of the sample, and the light absorption value of the sample is proportional to the concentration of the sample. Two spectrophotometer The light source: The ideal light conditions are as follows: to provide continuous radiation; light intensity is large enough; in the whole spectral region within the spectral intensity with wavelength changed obviously; the wide spectral range; the long service life, low price. The light source used in the visible and near infrared range is tungsten lamp. Now the most common one is Halogen lamp, which is halogen tungsten halogen lamp to improve the life of tungsten lamp. The applicable wavelength range is from 320 to 1100nm. Since the energy output fluctuation is four times the voltage fluctuation, the supply voltage must be stable. For ultraviolet light is deuterium lamp (Deuterium lamp), applicable wavelength range is 195 ~ 400nm, because deuterium lamp life is limited, domestic deuterium lamp life only five hundred hours or so, should pay attention to save lights. The monochromator: Is the heart of the monochromator spectrophotometer, its role is to mix the light from the light source into monochromatic light and can freely change the wavelength. Its main components and functions are: (1) inci

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