蛋白质提取相关（Protein extraction correlation）.docVIP

蛋白质提取相关（Protein extraction correlation） The pH value of concentrated and separated gum is different, the former is mainly concentrated utility, the latter is charge utility and molecular sieve effectiveness. The effect of concentrated mainly in concentrated gum, concentrated gum pH6.8, in the condition of pH, Cl ion buffer of HCl in almost all dissociation, the isoelectric point of Gly was 6, only a small number of negative ion dissociation, in the field of mobile speed is very slow. Acidic proteins are dissociated into negative ions at pH, and the migration rate of the three classes of ions is Cl. The protein Gly is generally used. After electrophoresis, the Cl ions migrate rapidly, leaving a low ionic concentration area thereafter. In the field of mobile Gly is very slow, caused by the lack of mobile ions, so the speed of ions on the formation of a lack of ions in the high-pressure area, in an area of high pressure negative ion all will accelerate the move, when moved to the Cl ion area, high voltage disappear, moving speed of protein to slow down when more stable after the establishment of the state, the protein sample is concentrated into a narrow layer in fast ion, and the protein according to the negative charge amount are arranged with the concentrated sample into the separation gel from concentrated gum, gum pH increased, Gly dissociation degree increased, migration rate increased, and because it is small, so more than all the protein molecules, on the heels of Cl ion, Cl ion mobility, low ion concentration no longer exist, forming a constant electric field strength, therefore, the protein samples in the separation gel Charge separation mainly depends on the molecular size and shape, pore separation gel has a certain size, the relative quality of different protein, blocking effect by receiving different, even if the electrostatic charge equal to that of the particles, also because the effect of the molecular sieve, the proteins of different sizes are separat