谷氨酸脱羧酶自身抗体对胰岛素分泌的影响（The effect of glutamic acid decarboxylase autoantibody on insulin secretion）.docVIP

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谷氨酸脱羧酶自身抗体对胰岛素分泌的影响（The effect of glutamic acid decarboxylase autoantibody on insulin secretion）.doc

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谷氨酸脱羧酶自身抗体对胰岛素分泌的影响（The effect of glutamic acid decarboxylase autoantibody on insulin secretion）

谷氨酸脱羧酶自身抗体对胰岛素分泌的影响（The effect of glutamic acid decarboxylase autoantibody on insulin secretion） The effect of glutamic acid decarboxylase autoantibody on insulin secretion 2010-01-13 Wang Jianmin, Zhou Zhiguang, Wu Hanwen [Abstract] Objective To investigate the mechanism of insulin deficiency in type 1 diabetes mellitus and the effect of GAD-Ab on the structure and function of pancreatic islet cells. Methods the radioligand modified method of screening in type 1 diabetic patients with GAD-Ab positive serum by affinity chromatography in the separation and purification of GAD-Ab from the serum, to observe the combination of GAD-Ab and beta cells by immunofluorescence; purified GAD-Ab and / or complement to human fetal islet cell culture system. The binding of GAD-Ab to beta cells, and the cultured islet cells insulin release were decreased; transmission electron microscopy, and reduce the group GAD-Ab beta cell cytoplasmic granules; cell membrane with complement group beta cell destruction. Conclusion serum GAD-Ab of type 1 diabetes mellitus can inhibit the synthesis and release of insulin in human fetal pancreatic islet beta cells in vitro, and has the effect of complement dependent cytotoxicity. Keywords type 1 diabetes mellitus; pathogenesis; glutamic acid decarboxylase autoantibody; Effectofglutamicaciddecarboxylaseautoantiboyon, insulin, secretion, Wang, Jianmin, Zhou, Zhiguang, Wu, Hanwen.Institute, of, Metabolism Endocrinology, Hunan, Medical, University, Changsha, [Abstract] Objective To study the mechanism of insulin insufficiency in type mellitus and to investigate 1 diabetes the effect of glutamic acid decarboxylase autoantiboy (GAD-Ab) on structure and function of pancreatic islet cells. Methods Radioligand assay of GAD-Ab was used to select the positive sera of GAD-Ab in type patients and affinity chromatography 1 diabetes was used to purify GAD-Ab from the positive sera. Indirect immunofluorescent technique was used to observe the binding of GAD-Ab to