高效液相色谱法测定芍甘颗粒剂中芍药甙含量（Determination of paeoniflorin content in Shao Gan granules by HPLC）.docVIP

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高效液相色谱法测定芍甘颗粒剂中芍药甙含量（Determination of paeoniflorin content in Shao Gan granules by HPLC）.doc

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高效液相色谱法测定芍甘颗粒剂中芍药甙含量（Determination of paeoniflorin content in Shao Gan granules by HPLC）

高效液相色谱法测定芍甘颗粒剂中芍药甙含量（Determination of paeoniflorin content in Shao Gan granules by HPLC） Determination of paeoniflorin content in Shao Gan granules by HPLC Update Date: 2010-11-19 Click: Zheng Chunying, Li Wenlan, Cao Xiuming, Yang Ming Abstract: a HPLC method for the determination of paeoniflorin in Shao Gan granules was developed. ODS column, methanol: water (40: 60) mobile phase, 254 nm detection, the average recovery rate was 96.47%, RSD 0.64%. Keywords HPLC; paeoniflorin; Determination, of, Paeoniflorin, in, Shaogan Granules by HPLC Zheng, Chunying, Li, Wenglan, Cao, Xiuming, et, Al Heilongjiang, Institute, of, TCM, Harbin 150036 Abstract A reversed-phase HPLC method was developed for the determination of Paeoniflorin in Shaogan granules. An ODS column was used and the mobile phase was methanol-water (40: 60). The detection wavelength was UV The average recovery was 254 nm. 96.47%. RSD=0.64% (n=5). Key words HPLC; Paeoniflorin Shao Gan Decoction originates from the treatise on febrile diseases, which is composed of peony and liquorice. Is the drug of choice for alleviating pain. Because of its many aspects of clinical efficacy, it is widely used in clinic. The preparation room of our hospital reformed the dosage form and made the granule without sugar, which was suitable for the prohibition of sugar patients. In order to control its quality better, HPLC method was used to determine paeoniflorin content. 1 experimental materials 1.1 drugs and reagents: methanol for analysis pure, water for ion exchange water, paeoniflorin standard products by China Pharmaceutical biological products inspection institute to provide. 1.2 apparatus: Japan SHIMADZU LC-10A high performance liquid chromatograph, SPD-10A ultraviolet visible detector, C-R 6A data processor. 2 experimental methods and results 2.1 chromatographic analysis conditions: ODS column; mobile phase MeOH-H2O (40: 60); flow rate 0.8 ml/min; column temperature 25 DEG C; paper speed 5 mm/min; detection wavelength 25