高浓度胰岛素、葡萄糖对hepg2细胞蛋白激酶c活性的影响（Effects of high concentration of insulin and glucose on protein kinase C activity in HepG2 cells）.docVIP

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高浓度胰岛素、葡萄糖对hepg2细胞蛋白激酶c活性的影响（Effects of high concentration of insulin and glucose on protein kinase C activity in HepG2 cells）.doc

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高浓度胰岛素、葡萄糖对hepg2细胞蛋白激酶c活性的影响（Effects of high concentration of insulin and glucose on protein kinase C activity in HepG2 cells）

高浓度胰岛素、葡萄糖对hepg2细胞蛋白激酶c活性的影响（Effects of high concentration of insulin and glucose on protein kinase C activity in HepG2 cells） Effects of high concentration of insulin and glucose on protein kinase C activity in HepG2 cells 2011-01-16 leaves Juan Li, Chen Jialun [Abstract] Objective To study the effect of high concentration of insulin and glucose on protein kinase C (PKC) activity in HepG2 cells. Methods with characteristics of insulin resistance and down-regulation of HepG2 receptor (DR-HepG2) to establish the cell model by 10-7mol/L insulin and glucose 25 mmol/L of untreated HepG2 (NDR-HepG2) and the influence of fine DR-HepG2 cell membrane PKC, cytosolic PKC activity. Results the PKC activity of membrane and cytoplasm of DR-HepG2 cells was higher than that of NDR-HepG2 cells without high concentration of insulin and glucose (P 0.05). 10-7mol/L stimulated NDR-HepG2 cells or DR-HepG2 cells at 30 and 5~10 minutes, and the activity of membrane PKC increased, and the activity of cytosolic PKC decreased (P 0.01). 25mmol/L glucose induced PKC cell membrane activity increased in two different phases, increased cytosolic PKC activity (NDR-HepG2 cell) or decreased (DR-HepG2 cell) (P 0.01) in HepG2. 10-7mol/L and 25 mmol/L glucose simultaneously stimulated cell and membrane and cytosolic PKC activity, which was basically the same as that of 25mmol/L stimulated by glucose alone. Conclusion the up regulation of PKC is related to the insulin resistance in DR-HepG2 cells. 10-7mol/L or 25 mmol/L glucose stimulated insulin under two different states of HepG2 cells caused by membrane PKC activity phase increased, but the effect on cytosolic PKC activity is not exactly the same; two simultaneous stimulation mainly through the mechanism of glucose mediated activation of PKC. [Keywords] protein kinase C, insulin, glucose, HepG2 cells Effects, of, high, concentrations, of, and, glucose, on, the, insulin, protein, kinase, C,, activities, of, HepG2, cells YE, Juan, LI, Guo, CHEN, Jia