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专注创新诚信和谐-百恩维生物
百恩维生物科技有限公司
专注 创新 诚信 和谐
专注 创新 诚信 和谐
仅供参考
深圳市百恩维生物科技有限公司
地址：深圳市宝安区67 区留仙一路高新奇战略新兴产业园二期1 号楼5 楼 邮政编码： 518100
电话： + 86 - 755 - 传真： + 86 - 755 - 网址：
百恩维生物科技有限公司
目录
一、慢病毒载体系统 ·························································································3
二、慢病毒的优点 ····························································································4
三、293T （Biowit 货号：C0002）细胞培养···························································4
1. 293T 细胞培养特性··················································································4
2. 293T 细胞完全培养液···············································································5
3. 293T 细胞的复苏·····················································································5
4. 293T 细胞的传代·····················································································5
5. 293T 细胞的冻存·····················································································6
四、慢病毒包装·······························································································7
1. HET(Biowit 产品号：BW11002)转染293T 细胞 ·············································7
五、慢病毒滴度测定（荧光法）··········································································8
六、慢病毒感染目的细胞预实验··········································································9
七、慢病毒的储存、稀释 ··················································································10
八、使用安全注意事项 ·····················································································11
九、慢病毒操作注意事项··················································································11
附录··········································································
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