药用植物北五味子的组织培养-植物研究.PDF

　　　　　　　　　 BULLET IN OF BOTA NICA L RESEARCH 19 　3 1999 　7 Vol.19 　No.3 July, 　1999 1 1 1 2 陈雅君 　吴秀菊 　关政华 　杨永富 (, 　150030)　(2., 165023) 　　取北五味子带腋芽的嫩茎作外植体, 培养于附加不同种类和浓度激素的 MS 培养基上。 附国加6-BA2.0+ZT0.1m /L 时, 诱导芽的效果最佳; 附加6- BA2.0+NAA0.2+ZT0.1m /L 时, 芽的增殖率最好; 附加NAA0.2 m /L 时, 生根效 果最佳。当苗高2.0 ～2.5cm, 根数达3 ～5 条时, 开瓶锻炼2 天, 移栽于消过毒的土 壤中, 10 天后, 成活率达100%。 　北五味子;外植体;组织培养。 THE TISSUE CULTURE OF DRUG PLANT—CHINESE MAGNOLIAVINE 1 1 1 2 Chen Ya-jun 　Wu Xiu-ju 　Guan Zhen -hua 　Yan Yon -fu (1.Northeast A ricultural University, Harbin 150030) (2.Xinlin Forestry Bureau, Heilon jian Province 165023) Abstract　The explants of Chinese ma noliavine tender stems w ith axillary buds are cultured in MS medium w ith addition of different concentration and kinds of hormone. The results show that sproutin of axillary bud is best on MS medium w ith addition of 6-BA2.0+ZT0.1m /L;and the multiplication of adventitious bud is best on M S medi- um w ith the addition of 6-BA2.0+NAA0.2 +ZT0.1 m /L;and the row th of roots is best on MS medium with addition of NAA0.2m /L.Seedlin s w ith rowin to 2.0 ～ 2.5cm and 3 ～5 roots are transplanted to the pots w ith disinfect soil after 2 days train- in .10 days later, the survival rate reaches 100%. Key words　Explant;Chinese ma noliavine;Tissue culture (S ch isandr a ch inensis (Turcz.)Baill) , , 、, 、, :1997-3-10 3 　　　　　　　　　　　　: 319 , 。, , 。