生长速度快且油脂产率高的湛江等鞭金藻诱变株的筛选.pdfVIP

微生物学通报 Jan. 20, 2015, 42(1): 142−147 Microbiology China © 2015 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.140320 研究报告 生长速度快且油脂产率高的湛江等鞭金藻诱变株的筛选 艾江宁 姚长洪 孟迎迎 褚亚东 曹旭鹏 薛松* (中国科学院大连化学物理研究所 海洋生物工程组 辽宁 大连 116023) 摘 要：【目的】筛选生长速度快的高产油湛江等鞭金藻诱变株。【方法】利用常压室温等离子体 射流诱变技术对湛江等鞭金藻进行诱变，通过 96 孔板、摇瓶和反应器培养对诱变株进行筛选。 【结果】传至第13 代，600 mL 反应器培养7 d ，诱变株IM110020 最大比生长速率、细胞密度和 −1 4 油脂产率可分别达0.72 d 、16 750×10 cells/mL 、109.8 mg/(L·d)，分别比野生株提高 12.5%、20.8% 和17.9%。【结论】获得了一株生长速度快且油脂产率高的诱变株IM110020 ，且经过多次传代性 质稳定。 关键词：常压室温等离子体，湛江等鞭金藻，生长速率，油脂产率 Screening of fast growth and high oil productivity mutants of Isochrysis zhangjiangensis AI Jiang-Ning YAO Chang-Hong MENG Ying-Ying CHU Ya-Dong * CAO Xu-Peng XUE Song (Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, China) Abstract: [Objective] Mutants of Isochrysis zhangjiangensis were screened for fast growth and high oil productivity. [Methods] Atmospheric and room temperature plasma jet mutation technique was used to mutate I . zhangjiangensis, and mutants were screened by different cultivation volume such as 96-well plates, flasks and bioreactors culture. [Results] After thirteen times passaged, mutant IM110020 was cultivated for 7 days in 600 mL bioreactor for evaluat