纤维素和木聚糖复合诱导合成木聚糖酶的研究-林产化学与工业.PDF

21 1 Vol.21 No. 1 2001 3 Chemistry and Industry of Forest Products Mar. 2001 1 2 2 刘超纲 , 勇 强, 余世袁 ( 1. 中南林学院工业学院, 湖南株洲412006; 2. 南京林业大学化工学院, 江苏 南京210037) LIU C G : ( Tr ichoder ma r eesei ) , , ; , ( 5 g/ L) , ( 4 g/ L) ( 1 g/ L) 45% : ; ; ; ; : Q 556 :A : 2001) [ 1~ 2] , , , , , , [ 3] , , , , , 1 1. 1 ( Trichoderm a reesei) Rut C30, :2000 01 26 : ( 1966 ) , , , , , 70 21 30 6~ 8 d , 4 1.2 ( Sigma ) [ 4] , , , : 110, 3%~ 4% , 121~ 125 1h : 5 g/ L( ) , 20 U/ g , pH 4. 8, 50 , 80~ 100 r/ min 24 h 1.3 [ 5] Mandels , 0. 05 mol/ L pH 4. 8, 2 d , 26~ 28 150 r/ min pH 7. 0 , ( 2000 r/ min, 10 min) , 1.4 : 0. 5 mL 1mL 0. 05 mol/ L 1%( w/ v) , 5030 min, 1min 1 mol ( ) , , 2 2. 1 , , 1, ( 1B) , , CMC 0. 6 U/ mL; , 72 h 16. 3 U/ mL, , , , ; , , , 2A , 54 h 23. 0 U/ mL, , 54 h 1 g/ L ( 2B ) , , 72 h 27. 7 U/ mL, CMC 1 , : 71 , 2B 1 2 Fig. 1 Comparison of purified cellulose w ith pulp Fig. 2 Effect of addition of purified cellulose on used as carbon source for enzyme production xylanase production xylanase; cel