灭活h37rv全菌体致敏的豚鼠模型制备-中国试验动物学报.docVIP

研究报告 结核病生物诊断试剂豚鼠模型建立 龚宝勇1，吴玉娥1，黄姣艳，，张钰1广东省实验动物监测所，广州，510663；，广州，510663. 广东瀚森生物药业有限公司，东莞，523808） 【摘要】 通过在豚鼠皮下剂量灭活H37Rv全菌体，制备标准结核菌素0.2 mg/mL，0.3 mg/mL和0.5 mg/mL0.2 mg/mL剂量组皮试后24 h和48 h红斑纵横径大于0.3 mg/mL和0.5 mg/mL红斑纵横径红斑纵横径≤0.05）；此外，灭活H37Rv全菌体致敏后，体重持续增加豚鼠注射部。应用mg/mL灭活H37Rv全菌体致敏。 【关键词】 H37Rv；豚鼠；模型动物；【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2014.010.000 Establishment of a guinea pig model for biological diagnostic reagent of tuberculosis GONG Bao-yong1, WU Yu-e1, HUANG Jiao-yan2, CUI Yu-hua2, ZHANG Yu1 （1. Guangdong Laboratory Animals Monitoring Institute，Guangzhou 510663, China. 2. Guangdong Handsome Bio-harmaceutical Co., Ltd，Dongguan 523808, China） 【Abstract】Objectiveo establish a guinea pig model for diagnostic reagent of tuberculosis. Methods By single or multiple subcutaneous injection of heat-killed H37Rv at different doses in the groin of guinea pigs to establish a model of positive response to 0.1 mL (5 IU) standard tuberculin (TB-PPD) skin test. Results Three doses of heat-killed H37Rv (0.2 mg/mL，0.3 mg/mL and 0.5 mg/mL) could be used to generate the model of biological diagnosis of tuberculosis. After 24 and 48 hours, the diameter of red spot by TB-PPD skin test was 15.4±2.3 mm when a dose of 0.2 mg/mL heat-killed H37Rv was administered for immunizing and allergizing the guinea pigs. The biggest red spot was induced at a dose of 0.3 mg/mL and 0.5 mg/mL. The test results showed that the immune response induced by multiple immunizing and allergizing guinea pigs was not significantly different than that induced by single immunizing injection, and the first skin test was better than the second, third and fourth skin test (P≤0.05). In addition, the body weight of the guinea pigs was still increasing after infection with heat-killed H37Rv, and ulcers occurred in the injection sites in some guinea pigs. Conclusions A single subcutaneous injection of 0.2