8 repeats of EPO mimetic peptide expression change part of the TIR（8重复EPO模拟肽表达改变行动的一部分）.docVIP

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8 repeats of EPO mimetic peptide expression change part of the TIR（8重复EPO模拟肽表达改变行动的一部分）.doc

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8 repeats of EPO mimetic peptide expression change part of the TIR（8重复EPO模拟肽表达改变行动的一部分）

8 repeats of EPO mimetic peptide expression change part of the TIR Papers to write network: Author: Han Wei Yan real Bob House, ZHAO Yong-Tong SHI Ji Zhang Ying from the [Keywords: translational initiation region Keywords: translation initiation region; SD sequence; EPO mimetic peptide; gene concatemer Abstract: Objective sequence observed by changing some of the translation initiation region of EPO analog peptide expression of the gene 8 repeats. Designed and synthesized some of the translation initiation region (translation the initiation region TIRDNA, sequence, this sequence into the pBSEPOM8 (including EPO analog 8 repeats peptide gene sequence with EcoRI and XbaI sites, and the correct recombinant plasmid was identified by DNA sequencing, and then EPO mimetic peptide gene 8 series body (including the alterations of the translation initiation region was cloned into the EcoRI and BamHI sites of the pBV220 carrier to induce the expression of results of DNA sequencing proved that the synthetic DNA sequence is properly inserted into the translation initiation region EPO mimetic peptide gene 8 repeats. the The series body the gene insertion pBV220 carrier at 42 ℃ induced bacterial lysis supernatant a new protein with a relative molecular mass of 22 × 103 (Mr), calculated molecular weight consistent with the theory of EPO mimetic peptide gene 8 repeats. proved by thin layer scanning the protein with about 15% of the total bacterial protein. Conclusion by changing some of the translation initiation region starting EPO mimetic peptide gene 8 repeats translation to obtain a higher level of expression, so that the original EPO mimetic peptide gene expression 8 repeats. Keywords: translation initiation region; SD sequence; EPO mimetic peptide; gene multiple repeats Abstract: AIM To observe effect of a synthesized DNA se-quence of translation initiation region (TIRon expression of8repeats of EOP mimetic peptide gene.METHODS A DNA sequence of translation in