低剂量阿霉素对胶质母细胞瘤放射增敏作用的研究.pdfVIP

exponential growth phase were incubated with ADR for 1h and concentration was 0.1ug/ml. Cells in fresh medium were then irradiated with graded doses(0, 2, 5 and 10Gy) of γ-ray using a cobalt-60 accelerator(75cGy/min) at certain time points(2, 4, 6, 8, 10 and 24h) after ADR treatment. The cells were then trypsinized and re-plated in specified numbers in drug-free medium to determine the colony-forming ability after 16-day incubation. The colonies with 50 cells were counted and the results were normalized by th